Computes metaspots in a given Seurat object containing spatial transcriptomics data.
This function is called by MetaspotsByGroups and should NOT be run directly!
Usage
ConstructMetaspots(
cur_seurat,
mode = "sum",
assay = "Spatial",
slot = "counts",
layer = "counts"
)
Arguments
- cur_seurat
A Seurat object
- mode
"sum" or "average"
- assay
Assay to extract data for aggregation. Default = 'Spatial'
- slot
Slot to extract data for aggregation. Default = 'counts'
- layer
Layer to extract data for aggregation. Default = 'counts'. Layer is used with Seurat v5 instead of slot.